| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2009B Blue |
0500-01SJB2U-0033 | n/a |
| A2 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2009B Garnet |
0500-01SJB2U-0032 | Austin Hayes |
| A3 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 09B Red |
0324-02SJB2U-0003 | Sias Reyneke |
| A4 | Emerald Force Soccer Club - 01 Emerald Force 09 Manchester City |
0214-01AB2U-1246 | Scott Marksberry |
| A5 | Nashville United Soccer Academy - 01 NUSA B09 Maroon |
0534-01AB2U-0117 | Giles Cheevers |
| A6 | Music City Soccer Club Music City FC 09/10 Boys |
0517-01AB2U-3468 | Juan Velasquez |
| A7 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B12U Black |
0521-01AB2U-2048 | Cesar Salinas Sr |