| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy 2009 Navy Boys |
0316-029B2U-7788 | B Wilkinson |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 09B Black |
0324-02SJB2U-0004 | Josue Fernandez |
| A3 | Clarksville Soccer Club Clarksville SC - B2009 Maroon |
0422-01AB2U-0001 | Alex Villa-merged |
| A4 | Jackson Soccer Club JSC 2009 Boys |
0609-01AB2U-0001 | Malang Jarju |
| A5 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B12U Red |
0521-01AB2U-2038 | Fulgence Letema |
| A6 | Tennessee Soccer Club TSC Williamson 2009 Boys SL Premier Blue |
0522-01AB2U-2015 | David Moss |