| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Arlington Soccer Academy - 01 Arlington Soc - B12U |
0627-01AB2U-0015 | Gregory Getman |
| A2 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2009B Yellow |
0500-01SJB2U-0034 | Cailin Harris |
| A3 | Chattanooga FC Academy Chattanooga FC Academy 2010 Navy Boys |
0316-029B1U-7785 | Nickson Odeny |
| A4 | Chattanooga FC Academy Chattanooga FC Academy 2009 White Boys |
0316-029B2U-7789 | Wesley Ford |
| A5 | Middle Tennessee Futbol Club - 01 MTFC Quest 2010 |
0525-01AB2U-0004 | Stephen Read |
| A6 | Upper Cumberland United Soccer Club - 01 UC United B 2009/2010 Purple |
0426-015B2U-1512 | Stephen Blake |
| A7 | White House Soccer Club - Select WHSC 09 Boys |
0427-02AB2U-0006 | Joseph Kozlik |
| A8 | Tennessee Soccer Club TSC Williamson 2009 Boys SL Premier White |
0522-01AB2U-2016 | David Moss |