| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy 2008 White Boys |
0316-022B3U-7792 | Gary Smith |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 08B SL |
0324-022B3U-0008 | James Lessentine |
| A3 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B13U Red |
0521-012B3U-2050 | Tommy Holt |
| A4 | Tennessee United Soccer Club TUSC 2009 Boys Red |
0424-01AB2U-0035 | Eduardo Carvacho |
| A5 | All-In FC TN All-In FC TN 08 Boys |
0443-01SLB3U-0043 | Bralen Clouse |
| A6 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2008B Yellow |
0500-01D2B3U-0030 | Juan Garcia |