| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Kings Hammer 2006 Premier Strikers |
0515-011B5U-0002 | Erich Delfs |
| A2 | Knoxville FC (Crush) Knoxville FC Crush 06B Premier |
0206-011B5U-0042 | Zinyor Babiker |
| A3 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B5U-0009 | Sunebari Ndam |
| A4 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B15U Black |
0521-011B5U-2047 | Jun Taulbee |
| A5 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B06 |
0213-01CB5U-0015 | Scott Muir |
| A6 | Tennessee Soccer Club TSC Williamson 2006 Boys Showcase |
0522-012B5U-0138 | Tre Arhagba |
| A7 | Tennessee Soccer Club TSC 2006 Boys Nike |
0522-011B5U-0085 | Tyler Kahn |
| A8 | Tennessee United Soccer Club TUSC 2006 Boys Red |
0424-011B5U-0011 | Robert DeWitt |