| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 06B SL |
0324-022B5U-0010 | Christopher Zeller |
| A2 | Tri-Cities United Soccer Club Tri-Cities United 06/07 Boys Premier |
0106-011B5U-0001 | Ovidiu Chirica |
| A3 | Knoxville Reds Football Club - 01 Knoxville Reds 06 Barcelona |
0290-01D2B5U-0008 | Gabriel Walker |
| A4 | Chattanooga FC Academy Chattanooga FC Academy 2006 Navy Boys |
0316-022B5U-7794 | Jose Francisco Ferraz |
| A5 | Kings Hammer 2006 Elite Strikers |
0515-012B5U-0006 | Raul Lopez |
| A6 | Tennessee United Soccer Club TUSC 2006 Boys Blue |
0424-012B5U-0020 | Lee Smith |