| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2005 Lobos Rush Premier |
0603-011B6U-0026 | Jason Haluska |
| A2 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2005B Blue |
0500-01D1B6U-0022 | Pablo Fuentes |
| A3 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 05B SL |
0324-021B6U-0011 | Andrew Wishart |
| A4 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 05 Boys Blue - SL |
0624-011B6U-2340 | Carl Schmitt |
| A5 | Nashville United Soccer Academy - 01 NUSA B05 Maroon |
0534-011B6U-0010 | Robert Stewart |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B16U Black |
0521-011B6U-2045 | Jun Taulbee |
| A7 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B05D1 |
0213-01CB6U-0008 | Scott Muir |
| A8 | Tennessee Soccer Club TSC Murfreesboro 2005 Boys Showcase |
0522-011B6U-0087 | Jason Finley |
| A9 | Tennessee Soccer Club TSC Williamson 2005 Boys Showcase |
0522-011B6U-0089 | James Pollock |