| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Wilson United Soccer Club EESC 2005 Boys Black |
0406-012B6U-1032 | Baylor McKay |
| A2 | Wilson United Soccer Club EESC 2005 Boys Red |
0406-013B6U-1033 | Christopher Prybilla |
| A3 | Knoxville FC (Crush) Knoxville FC Crush 05B Premier |
0206-012B6U-0009 | Vamuyan Sheriff |
| A4 | Knoxville Reds Football Club - 01 Knoxville Reds 05 Lions |
0290-01D2B6U-0011 | Patrick Fiveash |
| A5 | ALBION SC Nashville (Formerly Southern Premier Soccer) Southern Premier Soccer 05 Boys |
0540-02D2B6U-0019 | Brent Goulet |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B16U Red |
0521-012B6U-2046 | Jun Taulbee |
| A7 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B05D2 |
0213-01DB6U-0370 | Dino Sulejmanovic |