| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 05/06 Boys |
0443-01SLB6U-0045 | Aaron Alva |
| A3 | Chattanooga FC Academy Chattanooga FC Academy 2005 Navy Boys |
0316-023B6U-7796 | Alexander Harrison |
| A4 | FC Academy FC Academy - B16U |
0407-013B6U-1235 | Luis Altamirano |
| A5 | McMinn United Futbol Club McMinn United Futbol Club - B16U |
0308-013B6U-2090 | Seth Rucker |
| A6 | Middle Tennessee Futbol Club - 01 MTFC Quest 05/06 |
0525-013B6U-0002 | Ibrahim Nasr |
| A7 | Nashville United Soccer Academy - 01 NUSA B05 Navy |
0534-012B6U-0021 | Mirgen Bushati |
| A8 | Rhea County Football Club Play (RC3) Rc3 FC Raiders BU16 |
0322-01D3B6U-0002 | Zachary Daily |
| A9 | Tennessee United Soccer Club TUSC 2005 Boys White |
0424-013B6U-0041 | Bryson Lehn |