| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 02/03B |
0324-021B9U-0001 | James Lessentine |
| A2 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 02/03 Boys Blue- SL |
0624-011B0U-2337 | Andres Flores |
| A3 | Nashville United Soccer Academy - 01 NUSA B02 Maroon |
0534-011B9U-0012 | John Sintic |
| A4 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B19U Black |
0521-011B0U-2040 | Bret Farestad |
| A5 | Tri-Cities United Soccer Club Tri-Cities United 02/03 Boys Premier |
0106-011B0U-0003 | Matt Barry |
| A6 | Tennessee Soccer Club TSC Williamson 2002 Boys Showcase |
0522-011B0U-0101 | Christopher Price |