| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Armada Futbol Club Armada Futbol Club - G12U MC |
0436-019G2U-0038 | Mark Champion |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2010 Navy Girls |
0316-029G1U-7804 | Travis Markum |
| A4 | Nashville United Soccer Academy - 01 NUSA G09 Maroon |
0534-01AG2U-0118 | Wade Wonderlin |
| A5 | Tennessee Soccer Club TSC Williamson 2010 Girls Academy SL |
0522-01AG2U-2012 | Lance Mayes |