| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2009 Lady Lobos Rush Gold |
0603-01SG2U-0004 | Caroline Heblich |
| A2 | Arlington Soccer Academy - 01 Arlington Soc - G12U |
0627-01AG2U-0010 | Ryan Paule |
| A3 | Blount United Soccer Club Blount United Soccer Club - G12U |
0201-01AG2U-0240 | Darren Sutton |
| A4 | Chattanooga FC Academy Chattanooga FC Academy 2009 White Girls |
0316-029G2U-7806 | Robert Wright |
| A5 | Chattanooga FC Academy Chattanooga FC Academy 2009 Navy Girls |
0316-029G2U-7805 | Stephen Hirayama |
| A6 | Clarksville Soccer Club Clarksville SC - G2010 Maroon |
0422-01AG2U-0003 | Kelley Guth |
| A7 | Emerald Force Soccer Club - 01 Emerald Force 10 Chelsea |
0214-01AG2U-1243 | Jeff Grubb |
| A8 | Tennessee Soccer Club TSC Williamson 2009 Girls Academy |
0522-01AG2U-0024 | Lance Mayes |
| A9 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 09/10G Red |
0324-02SJG2U-0005 | Maegan Gibson |