| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Kings Hammer 2008 Lady Strikers Premier |
0515-01AG3U-0003 | Alan Oates |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2008 Navy Girls |
0316-02DG3U-7807 | Stephen Hirayama |
| A3 | Knoxville Reds Football Club - 01 Knoxville Reds 09 Ajax |
0290-01SJG2U-0004 | Derrick Long |
| A4 | Wilson United Soccer Club EESC 2008 Girls |
0406-012G3U-1037 | Mike Moore |
| A5 | Tri-Cities United Soccer Club Tri-Cities United 08 Girls Premier |
0106-012G3U-0005 | Leah Presley |