| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2007G Blue |
0500-01D2G4U-0026 | Eduardo Caturla |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2007 Navy Girls |
0316-02DG4U-7809 | Ron Senowech |
| A3 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 07G SL |
0324-021G4U-0006 | Luke Winter |
| A4 | Gallatin Rangers GSC Lady Rangers 07 |
0408-022G4U-0035 | Charles Shinton |
| A5 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - G14U Red |
0521-012G4U-2052 | Charles Bozman |
| A6 | Tennessee United Soccer Club TUSC 2007 Girls Blue |
0424-012G4U-0025 | Nate Sego |
| A7 | Nashville United Soccer Academy - 01 NUSA G07 Maroon |
0534-011G4U-0002 | Scott Davidson |